皮肤组织特异性表达hCTLA4-Ig转基因小鼠品系的建立

王勇 已出版文章查询
王勇
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1 王峰超 已出版文章查询
王峰超
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2 魏泓 已出版文章查询
魏泓
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weihong@mail.tmmu.com.cn
1 倪勇 已出版文章查询
倪勇
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1 吴军 已出版文章查询
吴军
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3 高翔 已出版文章查询
高翔
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1第三军医大学基础部实验动物学教研室,重庆,400038

2第三军医大学全军复合伤研究所,重庆,400038

3第三军医大学全军烧伤研究所,重庆,400038

4南京大学模式动物研究所,南京,210093


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为研究皮肤特异性高效表达hCTLA4-Ig分子对移植皮肤存活及受体免疫功能的影响,充分实现hCTLA4-Ig分子在皮肤移植中的免疫调控功能,利用K14(角蛋白14)基因启动子,构建了hCTLA4-Ig分子皮肤组织特异性表达载体,并通过受精卵原核显微注射技术制备了K14/hCTLA4-Ig转基因小鼠并建立了品系.通过RT-PCR和Northern blot分析表明,hCTLA4-Ig在转基因小鼠体内呈皮肤组织特异性高效表达;以GAPDH基因的表达量为内部参照分析表明,hCTLA4-Ig的表达水平在不同世代之间以及转基因个体的不同生命时相点之间保持相对恒定,说明皮肤组织特异性稳定表达hCTLA4-Ig的转基因小鼠品系已被建立.

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语种: 英文   

基金国家科技攻关项目(2001BA710B)

关键词hCTLA4-Ig 转基因小鼠 皮肤 角蛋白14


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